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Literature summary extracted from

  • Guan, W.; Bai, J.; Zhou, T.; Zhao, B.
    Active sites of N-acetylhexosamine 1-kinase from Bifidobacterium longum (2016), Wei Sheng Wu Xue Bao, 56, 68-77 .
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.162 D208A site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type Bifidobacterium longum subsp. Longum
2.7.1.162 D208E site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type Bifidobacterium longum subsp. Longum
2.7.1.162 D208N site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type Bifidobacterium longum subsp. Longum
2.7.1.162 F247A site-directed mutagenesis, the mutant shows altered pH optimum of pH 7.0 as compared to wild-type optimum of pH 7.5 Bifidobacterium longum subsp. Longum
2.7.1.162 H31A site-directed mutagenesis, the mutant shows altered pH optimum of pH 7.0 as compared to wild-type optimum of pH 7.5 Bifidobacterium longum subsp. Longum
2.7.1.162 H31V site-directed mutagenesis, the mutant shows altered pH optimum of pH 7.0 as compared to wild-type optimum of pH 7.5 Bifidobacterium longum subsp. Longum
2.7.1.162 I24A site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type Bifidobacterium longum subsp. Longum
2.7.1.162 I24V site-directed mutagenesis, the mutant shows altered pH optimum of pH 7.0 as compared to wild-type optimum of pH 7.5 Bifidobacterium longum subsp. Longum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.162 Mg2+ required for catalysis, optimum for wild-type enzyme is 5 mM, for mutants H31A and F247A 10 mM Bifidobacterium longum subsp. Longum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.162 Bifidobacterium longum subsp. Longum E8MF12
-
-
2.7.1.162 Bifidobacterium longum subsp. Longum ATCC 15707 E8MF12
-
-
2.7.1.162 Bifidobacterium longum subsp. Longum DSM 20219 E8MF12
-
-
2.7.1.162 Bifidobacterium longum subsp. Longum E194b E8MF12
-
-
2.7.1.162 Bifidobacterium longum subsp. Longum JCM 1217 E8MF12
-
-
2.7.1.162 Bifidobacterium longum subsp. Longum NCTC 11818 E8MF12
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.1.162 BLLJ_1622 locus name Bifidobacterium longum subsp. Longum
2.7.1.162 NahK
-
 Bifidobacterium longum subsp. Longum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.162 7
-
 mutant enzymes H31A, H31V, F247A, and I24V Bifidobacterium longum subsp. Longum
2.7.1.162 7.5
-
 wild-type enzyme Bifidobacterium longum subsp. Longum